Frequently Asked Questions (FAQ)

Below are answers to some of the more commonly asked questions. They are divided into several categories, depending on the subject of the question. If you do not see your question in the section you expect, please read through the entire list as your question may have been listed under another section. If you still do not see an answer to your question, please Contact Us, and we will answer it as best we can.

Accounts

Shipping

Orders

Payment

Stocks

iPCR

Exelixis Deficiencies

Accounts

Shipping

Orders

Billing/Payment

Stocks

Inverse PCR/Insertion Site

Exelixis Deficiencies


 

Accounts

Help, I forgot my password/username…

From the home page, click the “Request new password” link located beneath the login box. You will then be directed to enter your username or email address. Clicking on the “E-mail new password” button will cause an email to be sent to the email address associated with the username or the email address you entered. This email will contain a one-time only link that will allow you to login and change/reset your password. NOTE: If you have forgotten your username, this email will also contain your username on the first line.

I have a new shipping address. How do I change the old one to the new one?

You will have the opportunity to enter a new shipping address the next time you order. Since all of your shipping addresses are saved, they may be accessed via a drop-down menu while checking out when placing subsequent orders. Make certain that the new address is compatible with your FedEx account, or your shipment will not be delivered properly.

Some of my account information is incorrect. How can I fix this?

If the incorrect information is in regards to a billing address and you have an Individual Account, you must contact your Lab Account administrator. If you are the Lab Account administrator, login to your account, click on the "My account" link on the right side of the page, and click the "Edit" tab on the top of the page.

If the incorrect information is in regards to a shipping address, please Contact Us. For future orders, you will be able to enter the appropriate address when you are checking out. Since all of these addresses are saved, they may be accessed via a drop-down menu while checking out when placing subsequent orders.

If you are unable to correct the information using the information above, please Contact Us.

I’ve just started my own lab. Can I keep my old account and still order flies?

Yes, you can. Contact Us, and we'll start the process of changing your Individual Account into a Lab Account.

Shipping

I received a dead or destroyed stock. What do I do?

Contact Us with the details, and we'll get your replacement(s) to you as soon as possible.

When will I get my stock(s)?

Please see our Shipping Schedule.

How quickly can I get a stock?

We operate on a first-come, first-served basis, so we do not expidite shipments.  That being the case, generally if you place an order by the end of a Thursday, we will usually ship the order by the end of Wendesday the following week.

I need a permit to be shipped with my stocks. How do I get this to you?

You may either email us a pdf of the permit or fax it to us. If you choose to fax it, please Contact Us, and we'll be happy to send you the number.

My institution/department does not have a FedEx account/only uses carrier X. Will you ship using a different company/carrier X?

No, in order to streamline our operations, we only ship via FedEx. If your institution does not have a FedEx account, we suggest you create a personal FedEx account and have your institution reimburse you.

Customs delays that occur when shipping flies to my country using FedEx results in dead flies. Can you ship them to us via another method?

Yes, if this is a problem for you, please Contact Us, so we can make alternative arrangements.

Orders

How can I add/delete a stock from an order?

If you have placed an order that has not yet been collected and wish to add a stock to it, just place a new order and we will try to put the two orders together. You should mention this in the comment section of your new order and refer to the number of the older order.

If you need to delete a stock from an order that has not been collected, please Contact Us, and we will remove the item from your order.

Billing/Payment

Can I pay with a check or a Purchase Order?

No, now that we are able to accept credit card payments, we are no longer able to accept payment by any other form.

Stocks

I've searched for a stock and can't find it.  Do you have it?

If you have typed the stock number correctly (Please note that all stock numbers begin with a single letter (c, d, e or f) and are followed by five digits) and the search failed, we don't have that stock.  A substantial subset of the Exelixis insertions that are in FlyBase were culled from the collection prior to their being sent to us.  Since we do not have them, these stocks fail to yield a result on our search page.

A stock I'd like is listed as "Temporarily Unavailable". What does this mean, and can I get it?

Generally this means a stock is sick. Some of our stocks are chronically sick, and others will go through a difficult patch now and then. You should check back periodically to see if it becomes available. If, after a couple of months, it remains unavailable, it is one of our chronically ill stocks. If you'd still like us to ship it to you, please Contact Us, and we'll see what type of arrangement we may come to.

What is the genotype of my stock?

Genotypes would be best determined by consulting FlyBase , then:

1) Enter the Exelixis Stock ID into the “Enter text” of the QuickSearch field.
2) Click on the Exelixis entry (usually the first option, if there is a list).
3) Click on the designation entered into the FlyBase Genotype line.

If the transgene has been inserted into a known gene, it will appear in the Affected gene(s) line. If not, then it has not yet been characterized.

Why do I see multiple eye colors in my stock?

Multiple eye colors can arise for a number of reasons, among which are:

1) Flies continuously produce eye pigments and the eyes will darken with age. Freshly eclosed flies will look much lighter than their parents.

2) The transposons include the white mini-gene, which acts as though it is still on the X chromosome and engages in dosage compensation, even when inserted on an autosome (which is the case for a great majority of the collection). As such, males will most often have more eye color than females of the same age, keeping in mind these males are not hemizygous (as would be the as if the insert was on the X). The mechanisms underlying dosage compensation is still an area of active research, so the rationale as to why the transposons containing the white mini-gene act in such a manner is unclear.

3) Some fly lines are homozygous viable but may still include the balancer over which it was characterized, either CyO or TM6B, in the stock. Here, the homozygous viable flies will have two copies of the transgene, while the ones heterozygous over the balancer will have only one.

4) The line may be in the process of breaking down. If the insertion site is for some reason unstable, the transgene may “hop out” leaving a mixture of homozygous, heterozygous and white eyed flies.

Why do I see unexpected dominant markers in my stock?

Under construction

Inverse PCR/Insertion Site

I’d like to confirm the insertion site of a transposon by inverse PCR. Do you have a protocol/primer sequences that I can use?

Please visit our iPCR protocols page for further information.

I tried to confirm the insertion site in a stock and found that it differs from the annotated site. What’s going on here?

If have found the insertion site to be off by about 200 nt, and you are referring to the data provided in Supplementary Table 3 of Thibault, et. al, you should read the following paragraphs to more fully understand the data.  Otherwise, please Contact Us us and let us know what the problem is.

The keys to reading the data in Supplementary Table 3 are the following:

The first two letters refer to the element type. (XP, PB, RB or WH)
The second entry is the stock number. (one letter followed by five digits)
The third entry indicates the number of flanking sequences are associated with that particular stock.  In some cases this represents multiple inserts per stock, in others this may represent erroneously assigned sequence files (more on this later).
The fourth entry is the R3 coordinate of the insertion site.
The fifth entry is the chromosome arm.  U is unknown.
The sixth entry is the orientation of the element in the chromosome.
Finally, the flanking sequence data completes the set of data.  As a general rule, the first 200 nt precede the transposon while the remaining 201 nt are 3' to the insertion site.

All of this data is in the process of being refined.  Insertion sites should be considered approximate.  In the case of multiple flanking sequences for a given line, one should assume the existence of multiple inserts until proven otherwise.

Exelixis Deficiencies

I’d like to get an Exelixis deficiency, why can’t I find any in your stock list?

We do not carry any of the Exelixis deficiencies, however, they are available from the Bloomington Stock Center at: List of Exelixis Deficiencies.

I’d like to make a deficiency using Exelixis stocks. How do I go about this?

If you'd like to learn more about this, read Parks, et al. for a description of the stocks and crosses involved.

If you'd like to get some of the stocks necessary to generate deficiencies, please visit the Bloomington Stock Center's page that lists some of these stocks.